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| Registros recuperados : 30 | |
| 4. |  | ARAÚJO, J. F.; AZEVEDO, D. A. A. de; SOUSA, A. L. M. de; SOUZA, T. S. de; ANDRIOLI, A. Análise proteica bidimensional de antígeno do vírus Maedi-Visna. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 2., 2013, Sobral. Resumos... Sobral: Embrapa Caprinos e Ovinos, 2013. p. 59-60. (Embrapa Caprinos e Ovinos. Documentos, 109).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 5. | | ALBUQUERQUE, V. de A.; MEDEIROS, R. H. G.; PINHEIRO, R. R.; AZEVEDO, D. A. A. de; SOUSA, A. L. M. de; FACCIOLI-MARTINS, P. Y. Avaliação de ferramenta nanotecnológica para o controle de linfadenite caseosa; dados preliminares. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 6., 2017, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2017. p. 60-61. [PATRICIA YOSHIDA FACCIOLI MARTINS].| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 6. | | ARAÚJO, J. F.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; SANTOS, V. W. de S.; ALVES, F. S. F.; PINHEIRO, R. R. Avaliação da sensibilidade de testes de imunodiagnósticos para detecção de anticorpos contra o vírus da artrite encefalite caprina. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 3., 2014, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2014. p. 42.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | PEIXOTO, R. M.; ANDRIOLI, A.; PINHEIRO, R. R.; SOUZA, K. C. de; ARAÚJO, J. F.; SOUSA, A. L. M. de; LOPES, A. K. C.; SOUZA, S. C. R. Immune response dynamics of recent and chronic small ruminant lentivirus infection in the male reproductive system. Semina: Ciências Agrárias, v. 44, n. 1, p. 185-202, jan./fev. 2023. Título em português: Dinâmica da resposta imunológica da infecção recente e crônica por lentivírus de pequenos ruminantes no sistema reprodutor de machos.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | AZEVEDO, D. A. A.; SOUSA, A. L. M. de; ARAÚJO, J. F.; SANTOS, V. W. S. dos; ANDRIOLI, A.; PINHEIRO, R. R. Antígenos de cepas nacionais de lentivirus de pequenos ruminantes: análise protéica. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 1., 2012, Sobral. Resumos... Sobral: Embrapa Caprinos e Ovinos, 2012. p. 53-54. (Embrapa Caprinos e Ovinos. Documentos, 104).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | ARAÚJO, J. F.; AZEVEDO, D. A. A. de; SOUSA, A. L. M. de; SANTIAGO, L. B.; ANDRIOLI, A.; PINHEIRO, R. R. Análise imunogênica de antígeno do vírus Maedi-visna pelo Western Blot 2-D. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 2., 2013, Sobral. Resumos... Sobral: Embrapa Caprinos e Ovinos, 2013. p. 57-58. (Embrapa Caprinos e Ovinos. Documentos, 109).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | LOPES, A. K. C.; ARAÚJO, J. F.; PEIXOTO, R. M.; SOUSA, A. L. M. de; LIMA, A. M. C.; AMARAL, G. P.; VASCONCELOS, A. M. de; ANDRIOLI, A. Evaluation of maternal-filial stress in a dairy goat herd with small ruminant lentivirus infection in the Brazilian northeastern semiarid region. Journal of Veterinary Behavior, v. 46, 54–61, 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | ARAÚJO, J. F.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; SOUZA, T. S. de; ANDRIOLI, A.; PINHEIRO, R. R. Titulação de cepas do vírus da Artrite Encefalite Caprina isolados do estado do Ceará. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 1., 2012, Sobral. Resumos... Sobral: Embrapa Caprinos e Ovinos, 2012. p. 13-14. (Embrapa Caprinos e Ovinos. Documentos, 104).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | SOUSA, A. L. M. de; ARAÚJO, J. F.; AZEVEDO, D. A. A. de; SOUZA, T. S. de; ANDRIOLI, A.; PINHEIRO, R. R. Titulação de lentivírus de pequenos ruminantes através do cultivo de células de membrana nictitante caprina. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 1., 2012, Sobral. Resumos... Sobral: Embrapa Caprinos e Ovinos, 2012. p. 55-56. (Embrapa Caprinos e Ovinos. Documentos, 104).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | PEIXOTO, R. M.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; SILVA, A. A. de S.; AGUIAR, T. D. de F.; SOUSA, A. L. M. de. Perspectivas do uso de fitoterápicos no tratamento do sêmen caprino infectado pelo vírus da Artrite Encefalite Caprina. Medicina Veterinária (UFRPE), v. 12, n. 3, p. 193-201, jul/set. 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | AZEVEDO, D. A. A. de; SOUSA, A. L. M. de; ARAÚJO, J. F.; MUNIZ, F.; MARANGUAPE, R.; ANDRIOLI, A.; CRUZ, J. C. M. da; PINHEIRO, R. R. Perfil eletroforético bidimensional do antígeno de lentivírus de pequenos ruminantes: dados preliminares. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 7.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 13., 2012, Maceió. Anais... Maceió: Sociedade Nordestina de Produção Animal, 2012. 3 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; ARAÚJO, J. F.; ANDRIOLI, A. A.; SIDER, L. H.; PINHEIRO, R. R. Perfil imunogênico bidimensional de antígeno da artrite encefalite caprina produzido com cepa nativa do estado do Ceará. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 2., 2013, Sobral. Resumos... Sobral: Embrapa Caprinos e Ovinos, 2013. p. 63-64. (Embrapa Caprinos e Ovinos. Documentos, 109).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | SOUSA. A. L. M. de; AZEVEDO, D. A. A. de; AZEVEDO, D. A. A. de; ARAÚJO, J. F.; ANDRIOLI, A.; SIDER, L. H.; PINHEIRO, R. R. Perfil proteico bidimensional de antígeno da Artrite Encefalite Caprina produzido a partir de isolados do Estado do Ceará. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 2., 2013, Sobral. Resumos... Sobral: Embrapa Caprinos e Ovinos, 2013. p. 61-62. (Embrapa Caprinos e Ovinos. Documentos, 109).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | AZEVEDO, D. A. A. de; ARAÚJO, J. F.; SOUSA, A. L. M. de; DIAS, R. P.; SOUZA, T. S. de; ANDRIOLI, A.; PINHEIRO, R. R. Produção de antígeno de lentivírus de pequenos ruminantes através da cultura celular de membrana nictitante caprina. Revista Brasileira de Medicina Veterinária, v. 37, n. 4, p. 316-320, out./dez. 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | AZEVEDO, D. A. A. de; SANTOS, V. W. S. dos; SOUSA, A. L. M. de; PEIXOTO, R. M.; PINHEIRO, R. R.; ANDRIOLI, A.; TEIXEIRA, M. F. da S. Small ruminant lentiviruses: economic and productive losses, consequences of the disease. Arquivos do Instituto Biológico, v. 84, p. 1-10, e0552016, 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | PEIXOTO, R. M.; ANDRIOLI, A.; SANTOS, D. O.; PINHEIRO, R. R.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SILVA, D. F.; DAMASCENO, E. M.; TEIXEIRA, M. F. da S. Avaliação da toxicidade de solvente de extratos vegetais com ação antiviral em sêmen caprino refrigerado. Acta Scientiae Veterinariae, v. 45, n. 1487, p. 1-8, 2017. [ALICE ANDRIOLI PINHEIRO].| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | AZEVEDO, D. A. A. de; ARAÚJO, J. F.; LIMA, C. C. V. de; SOUZA, T. S. de; SOUSA, A. L. M. de; ANDRIOLI, A.; CRUZ, J. C. M. da; PINHEIRO, R. R. Análise protéica e imunogênica de antígenos de lentivírus de pequenos ruminantes. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 7.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 13., 2012, Maceió. Anais... Maceió: Sociedade Nordestina de Produção Animal, 2012. 3 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 30 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/09/2017 |
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Data da última atualização: |
22/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
PEIXOTO, R. M.; ANDRIOLI, A.; SANTOS, D. O.; PINHEIRO, R. R.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SILVA, D. F.; DAMASCENO, E. M.; TEIXEIRA, M. F. da S. |
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Afiliação: |
Renato Mesquita Peixoto, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brasil; ALICE ANDRIOLI, CNPC; DIONES OLIVEIRA SANTOS, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; Juscilânia Furtado Araújo, UECE - Fortaleza, CE, Brasil; Ana Lídia Madeira de Sousa, UECE - Fortaleza, CE, Brasil; Davi Faria Silva, Instituto Superior de Teologia Aplicada (Faculdades INTA) - Sobral, CE, Brasil; Edgar Marques Damasceno, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; Maria Fátima da Silva Teixeira, UECE - Fortaleza, CE, Brasil. |
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Título: |
Avaliação da toxicidade de solvente de extratos vegetais com ação antiviral em sêmen caprino refrigerado. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 45, n. 1487, p. 1-8, 2017. |
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Idioma: |
Português |
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Notas: |
[ALICE ANDRIOLI PINHEIRO]. |
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Conteúdo: |
[Evaluation of Solvent Toxicity of Plant Extract with Antiviral Action in Refrigerated Goat Semen]. Background: Caprine Arthritis Encephalitis Virus have been detected in sperm of breeding goats causing economic losses. In order to control the virus, researches aiming to identify natural extracts with potential antiviral effects are performed. However, aqueous or ethanolic extracts must be diluted in dimethyl sulfoxide (DMSO), which is a substance with unknown effects in sperm quality when present in diluting media. Therefore, this study aimed to evaluate sperm viability of refrigerated caprine semen diluted in media containing DMSO. This was performed to provide data that aid in researches involving the use of this component with natural extracts that may inactivate the caprine lentivirus in sperm. Materials, Methods & Results: The experiment was performed at the Laboratory of Seminal Technology in Embrapa Goats and Sheep in the city of Sobral, Brazil. Sperm viability was assessed in caprine semen refrigerated in two dilution media with crescent concentrations of DMSO. Sperm samples of five goats seronegative for the caprine lentivirus were pooled and diluted in minimal essential medium (MEM) enriched with glucose at 0.01 M added of crescent concentrations of DMSO (0%, 1.5%, 1.75%, 2.0%, 2.25% and 2.5%). The same breeders provided the pool of sperm to test Tris added 2.5% of egg yolk and the same concentrations of DMSO previously mentioned. Treatments were refrigerated at 7°C and evaluated up until four h after DMSO addition. Individual progressive motility (MIP), sperm vigor (V), percentage of spermatozoa reactive to hypoosmotic test (HO) and morphologically normal (NOR) were evaluated. IPM, vigor and NOR remained within normal standards for the caprine species in all treatments test. Percentage results of spermatozoa reactive to hypoosmotic was higher in Tris yolk with values ranging between 34.66% to 46.33%. Sperm vigor was positively correlated (r = 0.85) with IPM in the MEM diluted pool of sperm. In Tris yolk, vigor and hypoosmotic test correlated moderately (r = 0.63, r = 0.54, respectively) with IPM. Tris yolk medium added DMSO presented the highest percentage of reactivity to hypoosmotic test in all treatments when compared to MEM added DMSO. Discussion: The fact that DMSO is easily homogenized in water, ethylic alcohol and most organic solvents favors its use in diluting natural extracts. These components are a possible source of products that inactivate caprine arthritis encephalitis virus in sperm, which is the key to promoting the safe use of genetic material of infected breeders, in addition to commercial use of germplasm. In this study, there was no interference of DMSO in the analyzed parameters when added in a maximum concentration of 2.5% to MEM and Tris yolk, which is in accordance with standard values for goats. In addition, Tris yolk may promote greater protection to the membrane of sperm cells, which was demonstrated by hypoosmotic test. This medium could be ideal to be used in new methodologies that incorporate DMSO. In conclusion, DMSO added to dilution media Tris yolk and MEM did not interfere with the quality of refrigerated caprine sperm, which maintained viability. These results indicate that this substance did not present harmful effects to the genetic material, promoting the use as solvent of extracts from plant compounds with potential anti-viral effect. The information in this study may aid new research performed in this area. Menos[Evaluation of Solvent Toxicity of Plant Extract with Antiviral Action in Refrigerated Goat Semen]. Background: Caprine Arthritis Encephalitis Virus have been detected in sperm of breeding goats causing economic losses. In order to control the virus, researches aiming to identify natural extracts with potential antiviral effects are performed. However, aqueous or ethanolic extracts must be diluted in dimethyl sulfoxide (DMSO), which is a substance with unknown effects in sperm quality when present in diluting media. Therefore, this study aimed to evaluate sperm viability of refrigerated caprine semen diluted in media containing DMSO. This was performed to provide data that aid in researches involving the use of this component with natural extracts that may inactivate the caprine lentivirus in sperm. Materials, Methods & Results: The experiment was performed at the Laboratory of Seminal Technology in Embrapa Goats and Sheep in the city of Sobral, Brazil. Sperm viability was assessed in caprine semen refrigerated in two dilution media with crescent concentrations of DMSO. Sperm samples of five goats seronegative for the caprine lentivirus were pooled and diluted in minimal essential medium (MEM) enriched with glucose at 0.01 M added of crescent concentrations of DMSO (0%, 1.5%, 1.75%, 2.0%, 2.25% and 2.5%). The same breeders provided the pool of sperm to test Tris added 2.5% of egg yolk and the same concentrations of DMSO previously mentioned. Treatments were refrigerated at 7... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial properties; Caprine arthritis encephalit virus; Diluente; Dimetilsulfóxido; Fitocomposto; Fitoterápico; MEM; Semen diluents; Semen preservation; Tris Gema; Tris yolk. |
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Thesagro: |
Caprino; Criopreservação; Extrato vegetal; Sêmen. |
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Thesaurus NAL: |
Brazil; dimethyl sulfoxide; Goats; Plant extracts. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164230/1/CNPC-2017-Avaliacao-da-toxicidade.pdf
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Marc: |
LEADER 04811naa a2200457 a 4500
001 2076169
005 2020-01-22
008 2017 bl uuuu u00u1 u #d
100 1 $aPEIXOTO, R. M.
245 $aAvaliação da toxicidade de solvente de extratos vegetais com ação antiviral em sêmen caprino refrigerado.$h[electronic resource]
260 $c2017
500 $a[ALICE ANDRIOLI PINHEIRO].
520 $a[Evaluation of Solvent Toxicity of Plant Extract with Antiviral Action in Refrigerated Goat Semen]. Background: Caprine Arthritis Encephalitis Virus have been detected in sperm of breeding goats causing economic losses. In order to control the virus, researches aiming to identify natural extracts with potential antiviral effects are performed. However, aqueous or ethanolic extracts must be diluted in dimethyl sulfoxide (DMSO), which is a substance with unknown effects in sperm quality when present in diluting media. Therefore, this study aimed to evaluate sperm viability of refrigerated caprine semen diluted in media containing DMSO. This was performed to provide data that aid in researches involving the use of this component with natural extracts that may inactivate the caprine lentivirus in sperm. Materials, Methods & Results: The experiment was performed at the Laboratory of Seminal Technology in Embrapa Goats and Sheep in the city of Sobral, Brazil. Sperm viability was assessed in caprine semen refrigerated in two dilution media with crescent concentrations of DMSO. Sperm samples of five goats seronegative for the caprine lentivirus were pooled and diluted in minimal essential medium (MEM) enriched with glucose at 0.01 M added of crescent concentrations of DMSO (0%, 1.5%, 1.75%, 2.0%, 2.25% and 2.5%). The same breeders provided the pool of sperm to test Tris added 2.5% of egg yolk and the same concentrations of DMSO previously mentioned. Treatments were refrigerated at 7°C and evaluated up until four h after DMSO addition. Individual progressive motility (MIP), sperm vigor (V), percentage of spermatozoa reactive to hypoosmotic test (HO) and morphologically normal (NOR) were evaluated. IPM, vigor and NOR remained within normal standards for the caprine species in all treatments test. Percentage results of spermatozoa reactive to hypoosmotic was higher in Tris yolk with values ranging between 34.66% to 46.33%. Sperm vigor was positively correlated (r = 0.85) with IPM in the MEM diluted pool of sperm. In Tris yolk, vigor and hypoosmotic test correlated moderately (r = 0.63, r = 0.54, respectively) with IPM. Tris yolk medium added DMSO presented the highest percentage of reactivity to hypoosmotic test in all treatments when compared to MEM added DMSO. Discussion: The fact that DMSO is easily homogenized in water, ethylic alcohol and most organic solvents favors its use in diluting natural extracts. These components are a possible source of products that inactivate caprine arthritis encephalitis virus in sperm, which is the key to promoting the safe use of genetic material of infected breeders, in addition to commercial use of germplasm. In this study, there was no interference of DMSO in the analyzed parameters when added in a maximum concentration of 2.5% to MEM and Tris yolk, which is in accordance with standard values for goats. In addition, Tris yolk may promote greater protection to the membrane of sperm cells, which was demonstrated by hypoosmotic test. This medium could be ideal to be used in new methodologies that incorporate DMSO. In conclusion, DMSO added to dilution media Tris yolk and MEM did not interfere with the quality of refrigerated caprine sperm, which maintained viability. These results indicate that this substance did not present harmful effects to the genetic material, promoting the use as solvent of extracts from plant compounds with potential anti-viral effect. The information in this study may aid new research performed in this area.
650 $aBrazil
650 $adimethyl sulfoxide
650 $aGoats
650 $aPlant extracts
650 $aCaprino
650 $aCriopreservação
650 $aExtrato vegetal
650 $aSêmen
653 $aAntimicrobial properties
653 $aCaprine arthritis encephalit virus
653 $aDiluente
653 $aDimetilsulfóxido
653 $aFitocomposto
653 $aFitoterápico
653 $aMEM
653 $aSemen diluents
653 $aSemen preservation
653 $aTris Gema
653 $aTris yolk
700 1 $aANDRIOLI, A.
700 1 $aSANTOS, D. O.
700 1 $aPINHEIRO, R. R.
700 1 $aARAÚJO, J. F.
700 1 $aSOUSA, A. L. M. de
700 1 $aSILVA, D. F.
700 1 $aDAMASCENO, E. M.
700 1 $aTEIXEIRA, M. F. da S.
773 $tActa Scientiae Veterinariae$gv. 45, n. 1487, p. 1-8, 2017.
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Embrapa Caprinos e Ovinos (CNPC) |
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